Histone H3 (D1H2) XP ® Rabbit mAb (HRP Conjugate) 12648S from Cell Signaling Technology

Product Specs
ItemHistone H3 (D1H2) XP ® Rabbit mAb (HRP Conjugate)
CompanyCell Signaling Technology
Price $450.00 Inquire
Catalog Number12648S
Quantity100 µl
ApplicationsW
Conjugate/TagHRP
ReactivityH, M, R, Mk
TargetH3
HostRabbit
NCBI Full Gene NameH3 clustered histone 1
NCBI Gene AliasesH3/A, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, HIST1H3A
ClonalityMonoclonal
Research AreaChromatin Regulation / Nuclear Function
IsotypeIgG
CloneD1H2
Molecular Weight17

Questions and Answers

Can this antibody be used to detect different isoforms of Histone H3 in my experiments?

Yes, the Histone H3 (D1H2) XP® Rabbit mAb detects endogenous levels of total Histone H3 protein, including isoforms H3.1, H3.2, and H3.3, as well as the Histone H3 variant CENP-A.

Is this antibody suitable for use in Western blotting applications?

The Histone H3 (D1H2) XP® Rabbit mAb has been shown to work effectively in Western blotting applications, as evidenced by its use in multiple scientific studies, including those that detail protein extraction and analysis methods.

What species can this antibody react with?

This antibody is reactive with several species, including Homo sapiens (human), Mus musculus (mouse), Rattus norvegicus (rat), and non-human primates.

Has this antibody been cited in any relevant scientific literature?

Yes, the Histone H3 (D1H2) XP® Rabbit mAb has been cited in at least 20 scientific articles, indicating its utility and relevance in various research contexts, including studies on muscle regeneration and cancer biology.

Cell Signaling Technology

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Phone: 978-867-2388
Fax: 866-432-6112
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Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Selenoprotein H Functions as a PPARα Coactivator to Link Selenium Homeostasis to Hepatic Lipid Metabolism and Protect against SteatohepatitisAdvanced ScienceFebruary 8, 2026Yuwei Zhang, Yuchen Wang, Binbin Li, Xin Li, Chenyu Liu, Yanhao Chen, Cheng Tian, Dongmei Wang, Xiaosong Gu, Chunping Jiang, et al.(GB111081, Servicebio), EP300 (57625S, Cell signaling), GAPDH (10494‐1‐AP, Proteintech), Histone H3 (12648S, Cell signaling), DYKDDDDK tag (20543‐1‐AP, Proteintech), HA (66006‐2‐IG, Proteintech), αSMA (GB111364,

(2) Serine ADPr on histones and PARP1 is a cellular target of ester-linked ubiquitylationNature chemical biologyNovember 1, 2025Kolvenbach A, Palumbieri MD, Colby T, Nadarajan D, Bode R, Matić I.(Cell Signaling Technology, 12364; 1:1,000), HRP-coupled anti-histone H3 (D1H2) (Cell Signaling Technology, 12648; 1:1,000), Anti-ubiquitin antibody clone VU-1 (Life Sensors, VU101; 1 µg ml−1). The following secondary

(3) Age-Dependent Histone Deacetylase 3 Regulation by βA3/A1-Crystallin and Inositol Hexaphosphate in Retinal Pigmented Epithelial Cells Reveals a Novel Pathway in Age-Related Macular DegenerationAging cellSeptember 1, 2025Chatterjee S, Ghosh S, Sin Z, Babu VS, Preval LV, Davis E, Tran N, Bammidi S, Gautam P, Hose S, et al.(Thermo Scientific, Cat # 21059), then reprobed with H3 antibody (Cell Signaling Technology, Cat #: 12648S) at room temperature for 2 h followed by secondary antibody incubation and development (Ghosh et al.

(4) Histone H4 acetylation differentially modulates proliferation in adult oligodendrocyte progenitorsJournal of Cell BiologyNovember 4, 2024David K. Dansu, Ipek Selcen, Sami Sauma, Emily Prentice, Dennis Huang, Meng Li, Sarah Moyon, Patrizia Casacciarabbit anti-H3K27me3 (1:2,000, C1541095; Diagenode, RRID:AB_1281338), rabbit anti-Total H3 (1:5,000, 12648; Cell Signaling, RRID:AB_2797978), rabbit anti-H4K5ac (1:2,000, 39699; Active Motif, RRID:AB_2793311),

(5) Redundant but essential functions of PARP1 and PARP2 in DNA ligase I-independent DNA replicationNucleic acids researchSeptember 23, 2024Bhandari SK, Wiest N, Sallmyr A, Du R, Tomkinson AE.(Cell Signaling, cat. no. 2148S, 1:1000); rabbit mAb anti-H3, HRP conjugate (Cell Signaling, cat. no. 12648S, 1:5000); Alexa Fluor 488 goat anti-mouse IgG2a (Invitrogen, cat. no. A21131); Alexa Fluor 568 goat anti-rabbit IgG (H + L

(6) Clinically Defined Mutations in MEN1 Alter Its Tumor-suppressive Function Through Increased Menin TurnoverCancer Res CommunJuly 1, 2023Duan S, Sheriff S, Elvis-Offiah UB, Witten BL, Sawyer TW, Sundaresan S, Cierpicki T, Grembecka J, Merchant JL.Cell Signaling Technology), Rabbit anti-Histone H3 HRP-conjugated antibody (1:10,000 dilution, catalog no. 12648, Clone D1H2, RRID: AB_2797978, Cell Signaling Technology). For quantitation, films were scanned, converted

(7) Targeting Pro-Survival Autophagy Enhanced GSK-3β Inhibition-Induced Apoptosis and Retarded Proliferation in Bladder Cancer CellsCurrent OncologyMay 28, 2023Yuko Shirono, Vladimir Bilim, Tsutomu Anraku, Hiroo Kuroki, Akira Kazama, Masaki Murata, Kaede Hiruma, Yoshihiko Tomita(84966), p-Beclin-1 (S93) (14717), LC3 (3868), GSK3β (12456), TFEB (4240), α-Tubulin (9099), Histone3 (12648), p-ULK1 (14202), ULK1 (8054), p-AMPKα (2535), AMPKα (5831). The dilution ratios of the primary antibodies

(8) The protective role of CD73 in periodontitis: preventing hyper-inflammatory fibroblasts and driving osteoclast energy metabolismFront Oral HealthJanuary 1, 2023Ramos-Junior ES, Dawson S, Ryan W, Clinebell B, Serrano-Lopez R, Russell M, Brumbaugh R, Zhong R, Gonçalves Fernandes J, Shaddox LM, et al.Catalase (1:1,000, #14097); HRP Conjugate β-Tubulin (9F3) (1:1,000, #5346); HRP conjugate H3 (1:1,000 # 12648), HRP conjugate β-Actin (1:1,000 # 12620); HRP Conjugate GAPDH (1:1,000, #3683); CD73 (1:1,000, #PA5-85958,

(9) Tumor-intrinsic PRC2 inactivation drives a context-dependent immune-desert microenvironment and is sensitized by immunogenic virusesThe Journal of clinical investigationSeptember 1, 2022Yan J, Chen Y, Patel AJ, Warda S, Lee CJ, Nixon BG, Wong EW, Miranda-Román MA, Yang N, Wang Y, et al.MABI0321-100I); anti-H3K27ac (1:4000, Abcam, catalog ab4729), anti–histone H3 (1:2000, CST, catalog 12648); anti–β-actin (1:5000, Proteintech, catalog 66009-1-Ig); anti–lamin B1 (1:2000, Proteintech, catalog

(10) Suppression of Sost/Sclerostin and Dickkopf‐1 Augment Intervertebral Disc Structure in MiceJournal of Bone and Mineral ResearchJune 1, 2022Tori Kroon, Neharika Bhadouria, Paul Niziolek, Daniel Edwards, Roy Choi, Erica L Clinkenbeard, Alexander Robling, Nilsson HolguinHRP‐tagged actin antibody (#A3854, Sigma‐Aldrich) and nuclear fractions to HRP‐tagged histone H3 (#12648, Cell Signaling). All blots were developed using Immobilon Luminata Forte (Sigma), and the images were

(11) MLL5 is involved in retinal photoreceptor maturation through facilitating CRX-mediated photoreceptor gene transactivationiScienceApril 15, 2022Zhang X, Zhang BW, Xiang L, Wu H, Alexander SAS, Zhou P, Dai MZ, Wang X, Xiong W, Zhang Y, et al.RRID:AB_732924 H3K4me3 Mouse mAb,Abcam,Cat# ab1012; RRID:AB_442796 H3 Rabbit mAb,Cell Signaling Technology,Cat# 12648; RRID:AB_2797978 PKCα Mouse mAb,Santa Cruz,Cat# sc-8393; RRID:AB_628142 Rho Mouse mAb,Abcam,Cat# ab5417;

(12) De novo and cell line models of human mammary cell transformation reveal an essential role for Yb-1 in multiple stages of human breast cancerCell death and differentiationJanuary 1, 2022Lefort S, El-Naggar A, Tan S, Colborne S, Negri GL, Pellacani D, Hirst M, Gusterson B, Morin GB, Sorensen PH, et al.antibodies, such as anti-ACTIN (Santa Cruz, sc-1615, 1/10,000), anti-H3 (Cell Signaling Technology, 12648, 1/10,000), anti-RAS (Cell Signaling Technologies, 3339, 1/1,000), anti-YB-1 (Cell Signaling Technology,

(13) Effects of arsenic on the topology and solubility of promyelocytic leukemia (PML)-nuclear bodiesPloS oneJanuary 1, 2022Hirano S, Udagawa O.D15C11, rabbit, monoclonal); anti-GFP (Abcam, goat, polyclonal); HRP-conjugated anti-histone H3 (CST, #12648, rabbit, monoclonal); HRP-conjugated anti-α-tubulin (MBL, #PM054-7, rabbit, polyclonal); HRP-conjugated

(14) SUMO-Modification of Human Nrf2 at K110 and K533 Regulates Its Nucleocytoplasmic Localization, Stability and Transcriptional ActivityCellular Physiology and BiochemistryMarch 27, 2021Treniqka S. Walters, Deneshia J. McIntosh, Shalonda M. Ingram, Lakeisha Tillery, Evangeline D. Motley, Ifeanyi J. Arinze, Smita MisraCell signaling technologies; cat no: 70081S), anti-Histone H3 (1:1000 dilution, Cell signaling technologies: cat. no: 12648S) and anti-Fibrillarin (1:1000 dilution, Santa Cruz biotechnology, Dallas, TX; cat no: sc253970). Signals

(15) EZH2 Dynamically Associates With Non-coding RNAs in Mouse Hearts After Acute Angiotensin II TreatmentFrontiers in Cardiovascular MedicineFebruary 25, 2021Shun Wang, Ningning Guo, Shuangling Li, Yuan He, Di Zheng, Lili Li, Zhihua Wanggels and transferred onto PVDF membranes (Millipore). The blots were probed with antibodies for H3 (#12648), H3K27me3 (#9733), EZH2 (#5246), and GAPDH (#5174) from Cell Signaling Technologies, Inc. Protein signals

(16) Impact of WIN site inhibitor on the WDR5 interactomeCell reportsJanuary 19, 2021Guarnaccia AD, Rose KL, Wang J, Zhao B, Popay TM, Wang CE, Guerrazzi K, Hill S, Woodley CM, Hansen TJ, et al.RRID:AB_10695471 Rabbit monoclonal anti-Histone H3 (D1H2) XP (HRP conjugate),Cell Signaling Technology,Cat# 12648; RRID:AB_2797978 Rat monoclonal anti-HA (clone 3F10),Roche,Cat# 12013819001; RRID:AB_390917 Chicken

(17) MYC regulates ribosome biogenesis and mitochondrial gene expression programs through its interaction with host cell factor-1eLifeJanuary 8, 2021Popay TM, Wang J, Adams CM, Howard GC, Codreanu SG, Sherrod SD, McLean JA, Thomas LR, Lorey SL, Machida YJ, et al.211-032-171); rabbit anti-histone H3 (D1H2) XP monoclonal, HRP conjugated (Cell Signaling Technology Cat# 12648); rabbit anti-α-Tubulin (11H10) monoclonal, HRP conjugated (Cell Signaling Technology Cat# 9099); goat

(18) Single-cell analysis of autophagy activity in normal and de novo transformed human mammary cellsScientific ReportsDecember 1, 2020Sylvain Lefort, Sneha Balani, Davide Pellacani, Boris Guyot, Sharon M. Gorski, Véronique Maguer-Satta, Connie J. EavesUSA), anti-BECLIN1 (D40C5, 1/1,000, Cell Signaling Technologies, Leiden, The Netherlands), anti-H3 (12648, 1/10,000, Cell Signaling Technologies), anti-LC3B (3868, 1/1,000, Cell Signaling Technologies), or

(19) Small-molecule-induced polymerization triggers degradation of BCL6NatureDecember 1, 2020Słabicki M, Yoon H, Koeppel J, Nitsch L, Roy Burman SS, Di Genua C, Donovan KA, Sperling AS, Hunkeler M, Tsai JM, et al.anti-Hsp90 (Cell Signaling, 4874S), anti-HDAC1 (Cell Signaling, 2062S), anti-Histone H3 (Cell Signaling, 12648S), anti-eGFP (Cell Signaling, 2956), anti-V5-tag (ThermoFisher Scientific, MA5–15253), anti-Streptavidin

(20) Initiation of human mammary cell tumorigenesis by mutant KRAS requires YAP inactivationOncogeneFebruary 1, 2020Lefort S, Tan S, Balani S, Rafn B, Pellacani D, Hirst M, Sorensen PH, Eaves CJ.primary antibodies, i.e., anti-Actin (Santa Cruz, sc-1615, 1/10000), anti-H3 (Cell Signaling Technology, 12648, 1/10000), anti-Ras (Cell Signaling Technologies, 3339, 1/1000), anti-TAZ (Cell Signaling Technology,

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(1)

10/13/2020Great Histone H3 (D1H2) for Western Blot Sachin SamuchiwalI used this antibody as a loading control for nuclear fraction of THP1 cell samples treated with different compounds.

Histone H3 (D1H2) XP ® Rabbit mAb (HRP Conjugate) from Cell Signaling Technology

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Cell Signaling Technology

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Histone H3 (D1H2) XP ® Rabbit mAb (HRP Conjugate) from Cell Signaling Technology

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