COX IV (3E11) Rabbit mAb (HRP Conjugate) for Western Blot

October 26, 2020

Medicine
Harvard Medical School
Post-doctoral fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technologies

Product Name:

COX IV (3E11) Rabbit mAb (HRP Conjugate)

Catalog Number:

5247S

Clone Number:

N/A

I performed cell organelle fractionation and used COX IV antibody as a loading control for mitochodrial fraction. THP-1 cells treated with different stimulants were used to isolate the mitochondrial fraction.

Experimental Design and Results Summary

Applications

Western Blot

Sample

THP-1 cells

Primary Incubation

1:1000 dilution, 18 hours at 4 degrees with gentle shaking

Blocking Agent

5% non-fat dry milk in Tris buffer containing 0.1% Tween-20

Secondary Incubation

HRP-conjugated anti-rabbit antibody 1:2000 dilution, 1 hour at room temperature with gentle shaking

Tertiary Incubation

Not applicable

Detection

ECL based detection

Results Summary

Isolated mitochondrial fraction from different samples were enriched with COX IV protein suggesting high purity of fractionation.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Specificity

The Bad

Nothing

The Bottom Line

This antibody works great for western blot.

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