Fig 2: Receptor tyrosine kinases are regulated by the androgen receptor.Cells were deprived of androgens for 72 hours prior to stimulation with a synthetic androgen (10 nM R1881) for24 hours. LNCaP (A) and VCaP (B) cells were stimulated with R1881 in the presence and absence of tunicamycin (5 µg/ml) for 24 hours and protein lysates were harvested. The intensities of KLK3 and CaMKK2 were determined with densitometry, normalized to actin (LNCaP) or CoxIV (VCAP) and the protein amount in the vehicle treated cells was set to one. This experiment was repeated twice. C) LNCaP cells were stimulated with R1881 for 48 hours. Cell lysates were harvested and analyzed with a human phospho-RTK array. D) The signals arising from the array were determined with densitometry and normalized to the signals arising from the negative controls printed on the array. The RTK array data was obtained from a single experiment. Phosphorylated receptors are colour-coded in the figure.