Merck (Sigma-Aldrich)
Crystal violet solution 1% aqueous solution
V5265-500ML
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Crystal violet solution was used to stain Hela cells colonies after 15 days of treatment with DMSO and inhibitory drug.
Colony formation assay
Hela cells, 6 well plates, crystal violet, water and methanol
Crystal violet is a very toxic and known cancerogenic solution. Discard the wastes with attention!
100 cells/well of Hela cells were plates in a 6 well plate and growth for 15 days while treatment with DMSO (c-) and drug.Cells were washed with PBS 1x and fixated (10 minutes) with MeOH before staining with crystal violet 0,5%
Very fast staining of Hela cells and U2OS colonies (max 3 hours)
Toxic
The product works very well.