Great Signal from PE/Cy7 anti-mouse Ly-6A/E (Sca-1)

August 19, 2020

Experimental Hematology
Department of Biomedicine, University Hospital Basel
Graduate Student

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Company:

BioLegend

Product Name:

PE/Cy7 anti-mouse Ly-6A/E (Sca-1)

Catalog Number:

108114

Clone Number:

D7

Expression of Sca-1 analyzed on the mouse hematopoietic stem and progenitors from the bone marrow.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse BM cells

Primary Incubation

0.25 µg per million cells in 100 µl volume

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow Cytometry

Results Summary

Total bone marrow cells were erthrolyzed and stained with the primary antibody for 30 minutes at 4 degrees. Cells were washed once and expression was analysed with BD Fortessa Flow Cytometer.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good Signal.

The Bad

N/A

The Bottom Line

Recommended.

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