APC anti-mouse CD117 Works Well

August 17, 2020

Experimental Hematology
Department of Biomedicine, University Hospital Basel
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

APC anti-mouse CD117 (c-Kit)

Catalog Number:

105812

Clone Number:

2B8

Our lab focuses on Myeloid Malignancies and disorders. We study expression on lineage negative mouse HSPCs from bone marrow.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse BM cells

Primary Incubation

1.0 µg per million cells in 100 µl volume

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow Cytometry

Results Summary

Total bone marrow cells were erthrolysed and stained with the primary antibody for 30 minutes at 4 degrees. Cells were washed once and expression was analyzed with BD Fortessa Flow Cytometer.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good signal!

The Bad

N/A

The Bottom Line

Recommended.

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