Targetting Immune Cells in Peripheral Blood

August 20, 2020

Propriety Research Center for Healthy Lungs
Hunter Medical Research Institute
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

PE/Cyanine7 anti-human CD294 (CRTH2) Antibody

Catalog Number:

350118

Clone Number:

BM16

This anti-body works perfectly for identifying ILC2s in the peripheral blood.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Blood

Primary Incubation

5ul/million cellls for 30mins on ice

Blocking Agent

No blocking agent used

Secondary Incubation

No secondary incubation done

Tertiary Incubation

No tertiary incubation done

Detection

Flow Cytometer

Results Summary

Antibody binds nicely to the CRTH2 receptor on immune cells, specifically ILC2s, which are at very low number in the blood.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Works very well.

The Bad

None.

The Bottom Line

Works well to target CRTH2

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