Good ASR (IVD) CD4 Antibody

August 27, 2020

Pathology
Univ of Kentucky
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BD

Product Name:

CD4 FITC

Catalog Number:

340133

Clone Number:

SK3

Info:Supplier Page 

Browse Similar Products: CD4 Flow Cytometry Antibodies

We run patient whole blood samples, identifying lymphocyte subsets. We prefer to use ASR/IVD reagents.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human blood (lymphocytes)

Primary Incubation

No dilution. 15-20 minute incubation.

Blocking Agent

None

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometric (530/30 BP)

Results Summary

The results show excellent separation of CD4+ lymphocytes (helper T cells) using only 5uL of the reagent. The manufacturer recommends 20uL. Needless to say, each lab should do their own titration to ensure antigen saturation.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

ASR (IVD). Can be titrated down

The Bad

A little expensive

The Bottom Line

Good choice for helper T cell identification.

Share your experience with other scientists. Write a Review! »

Join the discussion