Rabbit Anti-GFAP Has Low Background Staining

August 25, 2020

BlueskyReddit
Angioscience
ECAS
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

DakoCytomation

Product Name:

Rabbit Anti-GFAP antibody

Catalog Number:

Z0334

Clone Number:

N/A

Good antibody to label astrocytes. Low background staining.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Adult mouse spinal cord

Primary Incubation

1:400, Overnight incubation at 4C in 2%Normal donkey serum in 0,3% triton-x100 in PBS

Blocking Agent

2%Normal donkey serum in 0,3% triton-x100 in PBS

Secondary Incubation

2h incubation at RT in 2%Normal donkey serum in 0,3% triton-x100 in PBS

Tertiary Incubation

None.

Detection

Confocal microscope.

Results Summary

This antibody detected astrocytes in the adult spinal cord with high specificity and low background.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Quality.

The Bad

None.

The Bottom Line

Recommendable antibody for researchers wanting to identify astrocytes.

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