Fisher Scientific
HIV-1 Core Antigen, Mouse anti-Human, PE, Clone: KC57, Beckman Coulter
CO6604667
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p24 is a mark for HIV infected cells, so we check p24 expression after HIV infection on CD4 T cells to see what percentage of cells were successfully infected.
Flow Cytometry
Human CD4 T cells
1:50, isolation buffer
Isolation buffer
No
BD C6 Plus
This p24 (KC57) ab works well, it shows about 5-20% of Cd4 T cells were successfully infected on Day 3, which means there are about 5-20% of CD4 T cells can produce mature HIV. The efficiency is enough for next step of experiment.
Easy to operate.
None found.
The ab works well, doesn't need an isotype, because the uninfected samples are the best isotype. Recommend.