Reliable Perm/Wash Buffer from BD Biosciences

COLUMBIA UNIVERSITY
M&I
Lab Director / Manager

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Company:

BD Biosciences

Product Name:

Perm/Wash Buffer

Catalog Number:

554723

Our lab studies various immune cells throughout the human body and one our techniques is to study cytokine production. In order to stain intracellularly for these cytokines, we use this Perm/Wash buffer to permeabilize the cells and allow the antibodies in.

Experimental Design and Results Summary

Application

Flow cytometry staining

Starting Material

Single cell human lymphocytes

Tips

Follow dilution guide.

Results Summary

We are able to stain for intracellular markers and keep the cells stable with this buffer.

DOI or PMID #

N/A

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Summary

The Good

Easy to use.

The Bad

None.

The Bottom Line

This is a great permeabilization buffer for human T cell intracellular staining.

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