Effective DNAse to Prevent Cell Clumping

COLUMBIA UNIVERSITY
M&I
Lab Director / Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

Sigma

Product Name:

Deoxyribonuclease I from Bovine Pancreas

Catalog Number:

DN25

Our lab processes large pieces of human tissue to extract single cell lymphocyte populations. During the processing, there is significant cell death, so we use DNAse to help prevent clumping of the dead cells.

Experimental Design and Results Summary

Application

DNAse

Starting Material

Human lymphocytes

Tips

Use at 0.1mg/ml concentration.

Results Summary

This DNAse is an easy addition to our buffers.

DOI or PMID #

N/A

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Summary

The Good

Works well.

The Bad

Precipitates at high concentration.

The Bottom Line

This DNAse works well to prevent clumping of cell isolates with dead cells.

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