Okay Signal with the GD2-FITC Antibody

July 15, 2020

Radiotherapy and Imaging
Institute of Cancer Research
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

FITC Anti-human Ganglioside GD2 Antibody

Catalog Number:

357313

Clone Number:

14G2a

Compared to GD2-PE, which is much brighter, the GD2-FITC antibody replicates the results but is the signal is weaker.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Neuroblastoma

Primary Incubation

1:20 dilution, 300,000 cells, 20 min incubation time, 4 C

Blocking Agent

Cell staining buffer (Cat 420201, Biolegend, USA)

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow Cytometry

Results Summary

Flow cytometry results paralled the alternatively labelled PE antibody (Biolgend, 357303), but with a lower staining intensity.

Image Gallery

Summary

The Good

Replicates brighter fluorophores

The Bad

Lower brightness

The Bottom Line

If you can use the PE-conjugated antibody it is better, but the FITC one works as an alternative.

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