Robust Results with PE-anti-human GD2

July 15, 2020

Radiotherapy and Imaging
Institute of Cancer Research
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE anti-human Ganglioside GD2 Antibody

Catalog Number:

357303

Clone Number:

14G2a

Very easy antibody to use, with little optimisation. Very bright signal.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Neuroblastoma

Primary Incubation

1:20 dilution, 300,000 cells, 20 min incubation time, 4 C

Blocking Agent

Cell staining buffer (Cat 420201, Biolegend, USA)

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow Cytometry

Results Summary

PE-anti-human GD2 showed strong staining of GD2 positive Kelly neuroblastoma cell lines, and low staining of GD2 negative SK-N-BE(2)C cell lines.

Image Gallery

Summary

The Good

Easy, Bright

The Bad

None

The Bottom Line

Good detection antibody for GD2+ cell lines

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