Merck
HiTrap® Protein G High Performance Columns
GE17-0404-01
Info: Supplier Page
Immunoglobulin G from antisera raised for specific antigen stimulation in rabbits in controlled experiments were purified with fast kinetics using HiTrap Protein G HP columns. The yield was 10 mg/mL of sera used. Purify was greater than 95%.
Separation and purification of IgG from Rabbit antiserum
2 ml of serum samples per HiTrap Protein G HP column
Make sure to saturate column with buffer before loading the samples. Use buffers with pH around 3 to 4 to elute IgG.
Overall, using HiTrap Protein G HP columns, IgG was eluted in fast kinetics. Elution can be done at 4mL per minute. The yield obtained was 10 mg/mL of elution.
Highly specific for separation/elution of IgG from the samples. Eluation can be achieved with fast kinetics (binding and elution)
None
Highly recommended for purification or separation of IgG or IgG subclass from the samples.