Great Lysis Buffer to Remove Red Blood Cells

COLUMBIA UNIVERSITY
M&I
Lab Director / Manager

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Company:

Gibco

Product Name:

ACK lysing buffer

Catalog Number:

A1049201

Our lab uses whole human tissues to extract lymphocytes for downstream applications. In order to remove RBC contamination, we treat the cells with this lysis buffer.

Experimental Design and Results Summary

Application

RBC lysis

Starting Material

Human lymphocytes

Tips

Should be stored in the dark

Results Summary

This lysis buffer (10 minutes at room temp) lyses all the RBCs.

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Summary

The Good

Works well

The Bad

None

The Bottom Line

This is a great buffer to remove RBCs from your cell mixture.

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