Great Transfer Kit for Western Blot

Johns Hopkins University School of Medicine
Department of Cell Biology
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Bio-Rad

Product Name:

TBT RTA Transfer Kit, LF PVDF

Catalog Number:

1704275

This kit is for fast, efficient protein transfer with the Trans-Blot Turbo rapid western blotting transfer system. Our lab purchased the equipment 3 years ago. The kit works very well. It only took 7-12 minutes to do the transfer for western blotting.

Experimental Design and Results Summary

Application

Protein transfer with the Trans-Blot Turbo rapid western blotting transfer system

Starting Material

Protein

Tips

Perform the transfer according to manufacturer's instructions. I usually transfer for 10 min.

Results Summary

The kit works very well. It only took 7-12 minutes to do the transfer for western blotting.

DOI or PMID #

N/A

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Summary

The Good

Reliable, high quality and save time

The Bad

Does not work well for protein with high molecular weight (larger than 200 kda)

The Bottom Line

I highly recommend this kit for Immunoblotting. The kit works very well.

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