Merck / Roche
Reverse Transcriptase AMV
10109118001
Studying gene expression using a Real-time PCR is mostly practiced activity in major molecular biology laboratories. for performing gene expression studies for the test samples require an efficient Reverstranscriptase to convert the mRNA into cDNA which can be used to analyze the gene expression. ROCHE reverse transcriptase is an efficient way to convert RNA into cDNA
Cloning, gene expression studies
Total RNA
Minimum of one microgram of total RNA should be used as a starting material
Good quality of cDNA is obtained at the end of the reaction. where low copy number genes are also able to be detected
N/A
Very efficient enzyme
We need to buy RNAse inhibitor seaperatly
I strongly recommend this enzyme if you are working with a low copy number gene expression.