PrestoBlue More Affordable Cell Viability Method

June 08, 2020

BlueskyReddit
UAB
Radiation Oncology
Research Scientist

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Company:

Life Technologies

Product Name:

PrestoBlue Cell Viability Reagent

Catalog Number:

A13261

PrestoBlue was used to measure cell viability of human pancreatic cells. Cells were treated with an agent in 2D monolayer format for 48 hours and measured by fluorescence.

Experimental Design and Results Summary

Application

Cell Viability

Starting Material

Human pancreatic cells

Tips

Make sure to have wells with only media to subtract background

Results Summary

Fluorescence values correlate with live cell viability and were used to plot against agent concentration. Values matched observations under microscope.

DOI or PMID #

N/A

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Summary

The Good

Quantifies cell viability with accuracy

The Bad

Must have empty wells to subtract background

The Bottom Line

PrestoBlue reagent is great for measuring cell viability by fluorescence or absorbable so it is compatible with most general use plate readers. Incubation for 15 minutes is sufficient.

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