Caspase-3 Antibody - Not Good for Flow

June 23, 2020

BlueskyReddit
Internal Medicine
East Tennessee State University
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Fisher Scientific

Product Name:

Caspase-3 Rabbit, Alexa Fluor 647, Clone: C92-605, BD

Catalog Number:

BDB560626

Clone Number:

C92-605, BD

Browse Similar Products: Caspase-3 Flow Cytometry Antibodies

Used to detect activ caspase-3 with or without CPT treatment in human PBMCs.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs

Primary Incubation

5ul in 100ul buffer for 45min

Blocking Agent

1X perm buffer

Secondary Incubation

No

Tertiary Incubation

No

Detection

BD C6 Plus

Results Summary

It shows lower after CPT treatment, which should be higher because CPT can induce apoptosis.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Easy to operate.

The Bad

Not good for flow.

The Bottom Line

Not recommend for flow, its result doesn't match what has been found.

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