Caspase 3 Antibody - Not Good for Flow

June 29, 2020

Internal Medicine
East Tennessee State University
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Santa Cruz Biotechnology

Product Name:

Caspase-3 Antibody (E-8) FITC

Catalog Number:

sc-7272

Clone Number:

E-8

Used to check caspase3 with or without CPT treatment in human PBMCs

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs

Primary Incubation

5ul in 100ul buffer for 45min

Blocking Agent

1X perm buffer

Secondary Incubation

No

Tertiary Incubation

No

Detection

BD C6 Plus

Results Summary

No positive population was found.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Easy to operate.

The Bad

No positive population was found.

The Bottom Line

Not recommend to use for flow, doesn't work at all.

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