RIP Antibody - Not Good for Flow

June 30, 2020

BlueskyReddit
Internal Medicine
East Tennessee State University
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Santa Cruz Biotechnology

Product Name:

RIP Antibody (C-12) FITC

Catalog Number:

sc-133102

Clone Number:

C-12

Used to detect RIP in human PBMCs with or without CPT treatment.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs

Primary Incubation

5ul in 100ul buffer for 45min

Blocking Agent

1X perm buffer

Secondary Incubation

No

Tertiary Incubation

No

Detection

BD C6 Plus

Results Summary

It shows lower after CPT treatment, which should be higher because CPT can induce apoptosis.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to operate.

The Bad

Not good for flow.

The Bottom Line

It goes against what has been found, not recommend for flow.

Share your experience with other scientists. Write a Review! »

Join the discussion