Great for Looking at IL-2 Expression in CD4+ Cells

June 03, 2020

BlueskyReddit
Oncology
Johns Hopkins School of Medicine
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Horizon™

Product Name:

PE-CF594 Rat Anti-Mouse CD25

Catalog Number:

562695

Clone Number:

PC61

Browse Similar Products: CD25 Flow Cytometry Antibodies

Since resting T cells express low levels of CD25, we use this to gate out the non-resting cells in our flow panel.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine Lymphocytes

Primary Incubation

1:1000

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow

Results Summary

This antibody works well if we want to specifically look at the CD4+s cells that have expression of CD25, specifically for CD4 Tregs. Our lab was interested in Treg in activated CD4s in the presence of Rapamycin therefore we stained this for our flow panel.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Definitely reliable and clean

The Bad

The price point is always high for antibodies

The Bottom Line

Definitely a must for gating out Tregs in your population.

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