BD Antibody Works Great for CD8+

Oncology
Johns Hopkins School of Medicine
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Horizon™

Product Name:

BV786 Rat Anti-Mouse CD8a

Catalog Number:

563332

Clone Number:

53-6.7

Browse Similar Products: CD8 Flow Cytometry Antibodies

Our lab is interested in CD8+ population in murine lymphocytes therefore when we harvest whole spleen, there is always a need for gating out CD8+ population in our flow panels.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine Lymphocytes

Primary Incubation

1:500

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow

Results Summary

It is always extremely simple to gate out the CD8+ population alongside with a Live/Dead fluorescent dye to specifically look at the live CD8+ populations. From there, you can look at other markers of your interest. For our project, we were interested in the effects of Rapamycin in culture and the effect it has on pS6 on the CD8s. It works well for gating out CD8s.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Clear population for CD8 gating

The Bad

Price is high

The Bottom Line

Our lab has been using BD antibodies and their CD8s, despite the clones, always are reliable.

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