Great for Gating out CD4+ Lymphocytes in Flow

June 01, 2020

BlueskyReddit
Oncology
Johns Hopkins School of Medicine
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Horizon™

Product Name:

BV786 Rat Anti-Mouse CD4

Catalog Number:

563331

Clone Number:

GK1.5

Browse Similar Products: CD4 Flow Cytometry Antibodies

The stain works really very well for gating out your CD4+ cells in your panel.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine Lymphocytes

Primary Incubation

1:500

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow

Results Summary

Our lab is interested in looking at CD4 population, specifically Treg in this project. Therefore it is very important to gate out CD4 from whole splenocyte when necessary. The stain works well with L/D flourescent dye to gate the live CD4+ cells. Population is clear and there needs to be dilution titration.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Clear population on flow

The Bad

Price is always high

The Bottom Line

This antibody works well for the price point. BD is always reliable and no risk of contamination.

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