VECTASHIELD® Antifade Mounting Medium with DAPI Gives Great Results

May 11, 2020

BlueskyReddit
UAB
Radiation Oncology
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Vector Laboratories

Product Name:

VECTASHIELD® Antifade Mounting Medium with DAPI

Catalog Number:

H-1200

For mounting tissue specimens for immunocytochemistry the VECTASHIELD® Antifade Mounting Medium with DAPI enables retention of fluorescence. There was no apparent loss of fluorescence after months of storage and the liquid did not introduce too many bubbles.

Experimental Design and Results Summary

Application

Immunocytochemistry (Immunofluorescence)

Starting Material

Mouse breast tumor tissue

Tips

Be careful to lay the coverslip with Vectashield onto the slide slowly so as to not introduce bubbles.

Results Summary

The fluorescence appeared to be adequate for microscopy and the DAPI stained well.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Very easy to use

The Bad

N/A

The Bottom Line

Use the Vectashield solution to mount tissue specimens with DAPI stain for microscropy. This product is highly recommended for fluorescence detection. Solution should be added with pipet rather than with the dropper to minimize wastage of liquid.

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