Great for Nonviable Cell Exclusion in Flow Cytometry

Humanitas Clinical and Research Center
Biomedical Sciences
Ph.D. Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen

Product Name:

eBioscience 7-AAD Viability Staining Solution

Catalog Number:

00-6993-50

The 7-AAD Viability Staining Solution was used as part of different multicolor flow citometry panel for nonviable cell exclusion.

Experimental Design and Results Summary

Application

Flow citometry

Starting Material

Human blood/bone marrow cells

Tips

It can be incubated together with antibodies, using the same staining buffer.

Results Summary

The 7-AAD Viability Staining Solution discriminated nonviable cells, allowing their exclusion from the analysed/sorted populations.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

No need to separately incubate the reagent (it can be added to the antibody mix)

The Bad

None at the moment

The Bottom Line

The 7-AAD Viability Staining Solution is an easy-to-use reagent that can be used and incubated in combination with fluorescent antibodies. The 7-AAD from Invitrogen performs as well as other more expensive competitors.

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