Excellent Labeling of RNA Transcripts in Live Cells

March 20, 2020

BlueskyReddit
Princeton
Molecular Biology
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

Click-iT™ RNA Alexa Fluor™ 488 Imaging Kit

Catalog Number:

C10329

Used a 1 mM pulse of EU for a 10 hour labeling experiment, revealed strong nucleolar staining of transcripts.

Experimental Design and Results Summary

Application

Fluorescent tagging

Starting Material

Live cells

Tips

Use EU at 1 -2 mM for 1-10 h

Results Summary

Results demonstrated excellent signal compared to background stains that was largely nucleolar. The kit instructions are very easy.

DOI or PMID #

N/A

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Summary

The Good

Total 1 hour detection process with great results

The Bad

No labeling of cytoplasmic mRNAs detected

The Bottom Line

Probably the best live cell labeling of mRNA transcripts that you could find and does not have the same photobleaching issues as FISH.

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