Good Blocking Reagent for TGFB1 Activity

Princeton
Molecular Biology
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Cayman Chem

Product Name:

LY2109761

Catalog Number:

15409

Was a suitable reagent for blocking TGFB1-SMAD signaling in human tissue culture experiments.

Experimental Design and Results Summary

Application

Flow cytometry

Starting Material

Human breast cancer cells

Tips

Dissolve compound in DMF

Results Summary

Results showed this compound could efficiently ablate TGFB-induced signaling in human cancer cells. Activity was observed over the 1-10 uM range.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Works well

The Bad

Not very avid, activity dropped off below 1 uM, activity variable between cell lines

The Bottom Line

This is probably the most efficient small molecule blocker of TGFB on the commercial market: it was nontoxic in cell culture at 6.6uM over 24 h, and was active at 6.6 uM.

Join the discussion