Great Antibody for Myelin Basic Protein Detection

February 21, 2020

Neuroscience
Wisconsin University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

ThermoFisher Scientific

Product Name:

MBP Polyclonal Antibody

Catalog Number:

PA1-10008

Clone Number:

Polyclonal

The goal of this experiment was to access demyelination in the course of disease progression in neurodegenerative diseases. This antibody showed excellent immunopositivity for myelin in the optic nerve.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse optic nerve

Primary Incubation

Diluted at 1:1000, overnight at 4°C

Blocking Agent

10% normal donkey serum + 0.5% triton X + 0.1M PBS

Secondary Incubation

Alexa 488 conjugated anti-chicken IgG 1:500, 1 hr at RT

Tertiary Incubation

N/A

Detection

Fluorescence

Results Summary

Robust fluorescence signals from the immunolabeling with the antibody highlighted myelination (green) in the optic nerve tissues.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very specific

The Bad

N/A

The Bottom Line

This is a good myelin marker for evaluating myelin pathology in the optic nerve.

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