Easy-to-Use CD103 Antibody from BD Biosciences

February 11, 2020

BlueskyReddit
Flowcore
University of Rochester
Clinical Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BV650 Mouse Anti-Rat Integrin αE2 (CD103)

Catalog Number:

745305

Clone Number:

OX-62

Browse Similar Products: CD103 Flow Cytometry Antibodies

The goal of the experiment is to quantify the CD103 population in rat spleen. This antibody is easy to use but does not give a distinct population.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Rat spleen

Primary Incubation

0.25ug per 100ul, 20 minutes on ice

Blocking Agent

Fc block

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

There is a low frequency of CD103 cells. The population is heterogeneous for this marker. There isn't a distinct population visible.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Easy to use

The Bad

Does not give a distinct population

The Bottom Line

This population could be rare in rat tissues to begin with. The problem may not be with the antibody.

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