A Good CD69 Antibody from Antibodies-Online

February 18, 2020

BlueskyReddit
Medicine
Brigham and Women's Hospital
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Antibodies-Online

Product Name:

anti-CD69 antibody (CD69) (FITC)

Catalog Number:

ABIN302016

Clone Number:

FN50

I stained human PBMCs to detect the activation marker, CD69 on CD8+ T cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human Peripheral Blood mononuclear cells.

Primary Incubation

1:100 at 4 degrees for 30 minutes

Blocking Agent

2% FBS in PBS

Secondary Incubation

Not applicable

Tertiary Incubation

Not applicable

Detection

Flow cytometry

Results Summary

This antibody worked well for flow cytometry and I was able to detect the levels of CD69 marker on CD8+ T cells.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

High specificity and no cross reactivity

The Bad

Nothing

The Bottom Line

Overall, this antibody works great for flow cytometry-based detection of CD69.

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