Anti-Mouse CD73 Antibody from Antibodies-Online

January 27, 2020

Medicine
Brigham and Women's Hospital
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Antibodies-Online

Product Name:

anti-Mouse (Murine) CD73 antibody

Catalog Number:

ABIN2662306

Clone Number:

TY-11-8

CD73 (ecto-5`-nucleotidase) is a 69 kD GPI-anchored surface protein. I tested the expression of this protein on CD4+ T cells in spleen using flow cytometry.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

1:100 at 4 degrees for 30 minutes

Blocking Agent

2% FBS in PBS

Secondary Incubation

Not applicable

Tertiary Incubation

Not applicable

Detection

Flow cytometry

Results Summary

The antibody was easier to optimize and gave specific reactivity.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

High specificity

The Bad

Nothing

The Bottom Line

Overall, it is a good antibody

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