Fluorophore/Clone Combo with Great Population Identification

February 11, 2020

BlueskyReddit
Surgery
NYU Langone Medical Center
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

APC anti-mouse CD4 Antibody

Catalog Number:

100516

Clone Number:

RM4-5

Bright fluorophore with clear identification of CD4 positive T cell population. Easy antibody handling and staining procedure.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine pancreas with orthotopically injected murine pancreas cancer cells

Primary Incubation

Incubated at 4C for 1h, dilution 1:500

Blocking Agent

1% FACS, anti-CD16/CD32, Mouse serum, Rat IgG

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Attune NxT

Results Summary

Very clear population separation with low laser voltages.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Straightforward staining procedure, nice separation of populations

The Bad

Waste of a bright fluorophore on fairly abundant surface marker

The Bottom Line

Reliable staining with clear population separation.

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