A Mounting Medium with DAPI

National Dairy Research Institute
Animal Biochemistry
Scientist

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Company:

Thermo Fisher Scientific

Product Name:

ProLong™ Gold Antifade Mountant with DAPI

Catalog Number:

P36931

Tissue sections from mice were subjected to immunofluorescence staining using the target primary antibody and anti-species Alexa fluor secondary antibody. These sections were nuclear stained and mounted in slides using ProLong™ Gold Antifade Mountant with DAPI. After the application of the mounting medium, the sections were covered using glass coverslip and kept overnight for drying. Further, the coverslips were sealed using nail-polish and examined under the fluorescent microscope.

Experimental Design and Results Summary

Application

Immunofluorescence staining

Starting Material

Mouse tissue, Primary antibody, Secondary antibody

Tips

Keep at room temperature and use in less quantity

Results Summary

The sections were mounted in this reagent shows nice, bright and non-fading signals as well as stained nucleus nicely.

DOI or PMID #

None

Image Gallery

Summary

The Good

This reagent gives the power to use the best long-lasting mounting medium with DAPI

The Bad

None

The Bottom Line

A mounting medium with DAPI which has the best performance.

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