Cell Signaling Technology
AMPKα
5832
D63G4
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Our lab is interested in looking at the levels of AMPK-a in comparison to the phosphorylation of AMPKa at the Thr172 site. Ny looking at the total amount, we are curious about whether there is a difference in total AMPK-a if we activate T cells in different conditions that changes the downstream mTOR signaling pathway.
Western Blot
Activated murine CD8+ T cells
1:5000 in 4 deg overnight
5% nonfat dry milk
Anti-rabbit IgG HRP-linked
N/A
ECL Pico
The antibody works consistently, showing that the levels remain almost the same during different conditions which is good for getting a ratio of the phospho-ampk and total ampk.
Consistency is the key for all experiments therefore this gives very consistent results
None
Cell Signaling always give promising results and consistency and it carries over throughout their antibodies.