AMPKalpha (D63G4) Rabbit mAb 5832S from Cell Signaling Technology

Product Specs
ItemAMPKalpha (D63G4) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number5832S
Quantity100 µl
ApplicationsW, IP
ReactivityH, M, R, Hm, Mk, B
HostRabbit
ClonalityMonoclonal
TargetAMPK-alpha1;AMPK-alpha2
NCBI Full Gene Nameprotein kinase AMP-activated catalytic subunit alpha 2, protein kinase AMP-activated catalytic subunit alpha 1
NCBI Gene AliasesAMPK, AMPK alpha 1, PRKAA, AMPK2, AMPKa1, AMPKa2
Research AreaMetabolism
CloneD63G4
Molecular Weight62 kDa

Questions and Answers

Can this antibody be used to study AMPK activation in human cells?

Yes, the AMPKα (D63G4) Rabbit mAb has been shown to effectively detect AMPKα protein levels in human cells, as evidenced by its use in various studies, including those investigating the effects of SGLT2 inhibitors on AMPK activation in renal cells ().

Is this antibody suitable for use in Western blotting applications?

The AMPKα (D63G4) Rabbit mAb is suitable for Western blotting, as it has been utilized in multiple studies for this application, including research on metabolic pathways and cellular responses to stress (, ).

Does this antibody cross-react with AMPKα2?

No, the AMPKα (D63G4) Rabbit mAb specifically detects endogenous levels of total AMPKα1 protein and does not cross-react with AMPKα2, making it a reliable choice for studies focused on AMPKα1 ().

What species can this antibody be used with?

The AMPKα (D63G4) Rabbit mAb is reactive with multiple species, including human, mouse, rat, non-human primate, hamster, and bovine, allowing for versatile applications in comparative studies across different models ().

Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) LGALS3BP Links Centrosomes and Mitochondria to Maintain Energetic Fitness and Restrain Compensatory Lipid Catabolic Reprogramming in Hepatocellular CarcinomaCancer Genomics ProteomicsApril 29, 2026Jun Eul Hwang, Hyun Jeong Shim, Hyun Jin Bang, Hyeon-Jong Kim, Sung Yun Jung, Ik-Joo Chung, Sang-Hee Cho, Dae-Hwan Kimfollowing primary antibodies: anti-phospho-AMPKα (Thr172), anti-total AMPKα, and anti-β-actin (#2535, #5832, and #4970; all from Cell Signaling Technology, Danvers, MA, USA). Detection was carried out using HRP-conjugated

(2) Transcriptional control of PPP2CB by YAP1 and TEAD1 mediates AMPK inhibition and mTORC1 activation in vascular smooth muscle cellsiScienceMarch 20, 2026Sanjana Kumariya, Joanna Stuck, Arturo Grano de Oro, Islam Osman(Cell signaling Technology, cat. #: 98840, rabbit, 1:1000), AMPKα1 (Cell signaling Technology, cat. #: 5832, rabbit, 1:1000), pACCS79 (Thermo Fisher Scientific, cat. #: PA5-17725, rabbit, 1:1000), pRaptorS792

(3) Copper deprivation reprograms antioxidant defense to suppress ferroptosis via SLC7A11Redox BiolMarch 19, 2026Qian Xue, Ziyuan Chen, Jiao Yang, Kaixuan Ren, Xiaofen Li, Xi Chen, Ding Yan, Yuan Wang, Daolin Tang, Jinbao Liu, et al.,Cell Signaling Technology,9282S phospho-AMPK,Cell Signaling Technology,2535S AMPK,Cell Signaling Technology,5832S CCS,Santa Cruz,sc-55561 GPX4,Abcam,ab125066 DHODH,Proteintech,14877-1-AP FTH1,Cell Signaling Technology,4393 AIFM2,Proteintech,20886-1-AP ACSL4,Proteintech,22401-1-AP Flag,Cell Signaling Technology

(4) Metformin Suppresses Glioblastoma Tumor Growth and Progression Through the AMPK/FoxO3a/Survivin AxisCellsFebruary 6, 2026Fabiola Cavaliere, Michele Pellegrino, Alessandro Cormace, Sofia Spadafora, Mariarosa Fava, Seung Ho Yang, Jung Eun Lee, Marta Claudia Nocito, Rosa Sirianni, Ivan Casaburi, et al.described [21]. The membranes were incubated with the following primary antibodies: anti-AMPK (D63G4; 5832S), p-AMPK (T172) (D4D6D; 50081S), AKT (11E7; 4685S), p-AKT (S473) (D9E; 4060S), FoxO3a (75D8; 2497S),

(5) Irisin alleviates hepatic steatosis by activating the autophagic SIRT3 pathwayChinese Medical JournalFebruary 5, 2026Ying Zhao, Jia Li, Anran Ma, Zhihong Wang, Yunzhi Ni, Di Wu, Yue Zhou, Na Zhang, Li Zhang, Yongsheng Chang, et al.Cell Signaling Technology), sirtuin 3 (SIRT3; ab217319, Abcam), p-AMPK (50081, Cell Signaling Technology), AMPK (5832, Cell Signaling Technology), p-mTOR (2974, Cell Signaling Technology), and mTOR (2983, Cell Signaling Technology

(6) Dynamic magneto-mechanical force in lysosomes induces durable macrophage repolarization for antitumor immunityCell ResearchFebruary 3, 2026Yingze Li, Mengge Zheng, Zhenyan Zhu, Yajuan Zhang, Peng Ning, Haotian Chen, Rui Gao, Chang Xu, Xueyan Wei, Yali Liu, et al.groups were lysed after stimulation, and whole-cell lysate extracts were incubated with 3 μg AMPK (CST, 5832 T) antibody or IgG control overnight at 4 °C. Extracts were then incubated with protein A/G Sepharose

(7) RCC1 Domain‐Containing Protein 1 Promotes Colon Cancer Malignant Progression by Activating Autophagy‐Dependent WNT5A Secretion in Cancer‐Associated FibroblastsJournal of the Royal Society of New ZealandFebruary 1, 2026Chao Liu, Sheng Xu, Yuanyuan Liu, Yuntian Tanganti‐mTOR (Cat# 2983, CST, 1:1000), anti‐phospho‐AMPKα (Thr172) (Cat# 2535, CST, 1:1000), anti‐AMPKα (Cat# 5832, CST, 1:1000), anti‐phospho‐ULK1 (Ser757) (Cat# 14202, CST, 1:1000), anti‐ULK1 (Cat# 8054, CST, 1:1000),

(8) Inhibiting SHP2 improves ventricular remodeling by restoring AMPK phosphorylation and mitochondrial homeostasisCell Communication and SignalingJanuary 7, 2026Qiao-Juan Shi, Wei-Qi Li, Ya-Nan Liu, Guo-Xuan Liu, Jia-Ning Zheng, Ming-Yang He, Chen-Lu Liu, Yu-Rou Wu, Jie Tong, Meng Zhang, et al.antibodies, including p-AMPK (1:1000; cat. no. 50081, Cell Signaling Technology), AMPK (1:1000; cat. no. 5832, Cell Signaling Technology), SHP2 (cat. no. 3397, Cell Signaling Technology), GAPDH (1:1000; cat. no.

(9) Cooperative CCL2/CCR2 and HGF/MET signaling enhances breast cancer growth and invasion associated with metabolic reprogrammingCancer Biology & TherapyDecember 31, 2025Wei Fang, Yuuka Kozai, Diana S. Acevedo, Rebecca Brodine, Haasini S. Gorrepati, Nizhoni Arviso, Paige Cote, Alala Thompson, Zachary Gerdes, Ashley Espinoza, et al.phospho-AMPKα Thr172 (Cell Signaling Technology cat no.2535S), AMPKα (Cell Signaling Technology cat no.5832S), phospho-AKT Ser473 (Cell Signaling Technology cat no.406S), AKT (Cell Signaling Technology cat no.4691S),

(10) Ribosomal protein control of hematopoietic stem cell transformation through regulation of metabolismCell ReportsDecember 23, 2025Bryan Harris, Dinesh K. Singh, Billy Truong, Michele Rhodes, Rachael Price, Susan Shinton, Monika Verma, Bridget Aylward, Shawn P. Fahl, Shanna R. Sprinkle, et al.report,Custom Polyclonal rabbit anti-Rpl22L1,This lab; this report,Custom Rabbit monoclonal anti-AMPK,Cell Signaling,5832S Rabbit monoclonal anti-pAMPK,Cell Signaling,– Mouse monoclonal anti-tubulin,Santa Cruz Biotech.,Sc-5286

(11) PFKFB3 ‐Inhibitor 3PO ‐Mediated Glycolytic Reprogramming Promotes Inflammatory Dental Pulp Repair: An In Vitro and In Vivo StudyInternational Endodontic JournalDecember 1, 2025Yanqiang Zhao, Yuqing Peng, Dan Wang, Lixin Zhang, Yijia Qiu, Jingyi Cui, Fei Xie, Ningxin Zhu, Man Qin, Yuanyuan WangCell Signalling Technology), phosphorylated p65 (#3033, 1:2000 dilution; Cell Signalling Technology), AMPK (#5832, 1:2000 dilution; Cell Signalling Technology), phosphorylated AMPK (#2535, 1:2000 dilution; Cell Signalling

(12) GDF6 Alleviates Pathological Cardiac Hypertrophy via AMPKα Signaling PathwayBiomedicinesNovember 29, 2025Quan Ren, Zhiwei Wang, Wei RenAbcam), anti-phospho-AMPKα (p-AMPKα, #2535; CST, Danvers, MA, USA), and anti-total-AMPKα (t-AMPKα, #5832; CST) at a dilution of 1:1000, and then incubated with horse radish peroxidase (HRP)-conjugated secondary

(13) Cepharanthine Induces Oxidative Stress and Apoptosis in Cervical Cancer via the Nrf2/Keap1 PathwayAntioxidants (Basel)November 1, 2025Ya-Hui Chen, Jyun-Xue Wu, Shun-Fa Yang, Tze-Ho Chen, Yun-Chia Wu, Tzu-Chi Lin, Yi-Hsuan Hsiao(D2E11) (#5023), cleaved caspase-3 (Asp175) (#9664), phospho-AMPKα (Thr172) (#2535), AMPKα (D63G4) (#5832), phospho-p53 (S15) (#9284), p53 (#9282), Nrf2 (D1Z9C) (#12721), Keap1 (D6B12) (#8047), NQO1 (A180)

(14) Context-specific applications of CARM1 inhibitors: functional profiles of EZM2302 and TP-064Mol MedOctober 31, 2025Yena Cho, Yong Kee Kim(Santa Cruz Biotechnology, Dallas, TX, USA, sc-47778), AMPK (Cell Signaling Technology, Danvers, MA, USA, #5832), p-AMPK (Cell Signaling Technology, #2535) CARM1 (Bethyl Laboratories, Montgomery, TX, USA, A300-421

(15) Rebamipide Induces Hair Regeneration Through EP4-Driven Lipid Metabolism RemodelingInternational journal of molecular sciencesOctober 18, 2025Feng C, Dong H, Jiang D, Gao Y, Gu X, Diao W, Zhou Y, Xu D, Li R, Wu L.anti-p62 (1:5000; HA721171, Huabio), anti-Phospho-AMPKα (Thr172) (1:1000; 50081, CST), anti-AMPKα (1:1000; 5832, CST), anti-Beclin-1 (1:1000; 3495, CST), anti-CPT1A (1:1000; 12252, CST), anti-ATGL (1:1000; 2439,

(16) Aging-rewired metabolic cues promote autophagy and senescence via DRAM1AutophagyOctober 14, 2025Jinghong Yang, Haobin Sun, Keqing Xu, Xiaomei Zhang, Mudan Huang, Guanghui Jin, Yasong Liu, Weizhao Chen, Shunan Lin, Juan Shen, et al.mAb,Cell Signaling Technology,2535T,WB 1:1000 PRKAA/AMPKα (D63G4) rabbit mAb,Cell Signaling Technology,5832S,WB 1:1000 RPS6KB/p70 S6 Kinase rabbit mAb,Selleck,F0011,WB 1:1000 Phospho-RPS6KB/p70 S6 Kinase (Thr389)

(17) Glutamate decreases oxidative stress and lipid droplet formation in astrocytesJournal of Cell ScienceOctober 1, 2025Luis Fernando Rubio-Atonal, Jinlan Chang, Julie Jacquemyn, Isha Ralhan, Iset Ilarraza, Maria S. Ioannoufor western blotting. Antibodies used were as follows: rabbit monoclonal anti-AMPKα (D63G4) (Cell Signaling Technology, 5832, RRID: AB_10624867; 1:1000), rabbit monoclonal anti-phospho-AMPKα Thr172 (40H9) (Cell Signaling Technology,

(18) Adiponectin Receptor Agonist Ameliorates Synaptic Dysfunction in 3xTg Alzheimer's Disease Mouse Model by Activation of AMPKCNS Neuroscience & TherapeuticsSeptember 22, 2025Jenna Bloemer, Priyanka D. Pinky, Vishnu Suppiramaniam, Miranda N. Reedantibodies,Primary antibodies,Primary antibodies,Primary antibodies AMPK,Rabbit,Cell Signaling Technology,5832,1:1000 pAMPK (Thr 172),Rabbit,Cell Signaling Technology,2531,1:500 GSKβ,Rabbit,Cell Signaling Technology,"12,456",1:1000 pGSK3β

(19) Nose‐to‐Brain Delivery of Acyl‐Ghrelin Peptide Gold Nanoconjugates for Treatment of Neurodegenerative DiseasesSmallSeptember 1, 2025Shunping Han, Rifka N. Utami, Yue Qin, Revadee Liam‐Or, Jemeen Sreedharan, Jeffrey S. Davies, Khuloud T. Al‐Jamalantibody (bs‐0467R‐BSS) was purchased from Stratech (UK). Phospho‐AMPKα (Thr172) (#2535), AMPKα (D63G4) (#5832), and HRP‐linked anti‐rabbit IgG (#7074S) were purchased from Cell Signaling (UK). Tris‐Buffered Saline

(20) Lysionotin attenuates bleomycin-induced pulmonary fibrosis by activating AMPK/Nrf2 pathwaySci RepAugust 25, 2025Xiaohua Zhang, Dayan Xiong, Lang Deng, Rui Qian, Siyuan Tang, Wei Liu, Yin Li, Lang Liu, Weixi Xie, Miao Linp-AMPK monoclonal antibody (1:1000, Cat# 50081 S, CST, USA), AMPK monoclonal antibody (1:1000, Cat# 5832T, CST, USA), HO-1 monoclonal antibody (1:2000, Cat# ab189491, Abcam, UK), NQO-1 monoclonal antibody(1:10000,

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1/3/2020Clear Antibody for AMPK-a Matt SunCell Signaling always give promising results and consistency and it carries over throughout their antibodies.

AMPKalpha (D63G4) Rabbit mAb

AMPKalpha (D63G4) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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AMPKalpha (D63G4) Rabbit mAb from Cell Signaling Technology

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