Very Good Staining for Mitochondrial Potential

January 14, 2020

BlueskyReddit
Johns Hopkins School of Medicine
Oncology
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

Tetramethylrhodamine, Ethyl Ester, Perchlorate (TMRE)

Catalog Number:

T669

We are interested in studying the mechanism of T cell differentiation through modulating cellular metabolism.

Experimental Design and Results Summary

Application

Flow Cytometry

Starting Material

Mouse T cells

Tips

Cell number and titration is very critical

Results Summary

Very good staining, but spectral spillover to lots of nearby channels

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Good signal

The Bad

Need careful titration and flow panel design to avoid spillover

The Bottom Line

Good tool but takes time to optimize.

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