Easy and Ready-to-Use Mounting Solution with DAPI

Johns Hopkins School of Medicine
Oncology
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

SlowFade™ Diamond Antifade Mountant with DAPI

Catalog Number:

S36964

We routinely perform confocal microscopy of cells on slides. The final step is to mount the samples to prevent photobleaching and allow proper imaging. This solution is ready to use mounting solution with DAPI, so you can forgo additional DAPI staining. Unlike other solutions, this mounting solution can be cured quickly and then proceed to imaging rather then curing overnight.

Experimental Design and Results Summary

Application

Confocal Microscopy

Starting Material

HEK293 T cells

Tips

Always use correct amount based on surface area to prevent bubbles.

Results Summary

Applied solution as final step of staining then sealed with nail polish after dropping cover slip. Immediately acquired images.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Ready to use and fast mounting solution.

The Bad

None really

The Bottom Line

Great mounting solution if need to image without overnight curing step.

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