Strong Staining in Mouse Embryonic Pancreas

October 08, 2019

Molecular Genetics
University of Toronto
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Recombinant Anti-Insulin antibody

Catalog Number:

ab181547

Clone Number:

EPR17359

Looking at pancreatic development in mouse embryos, and how different endocrine cell populations develop. Used this antibody to specifically assess the development of insulin-positive beta cells.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse pancreas

Primary Incubation

1:500 dilution, incubated overnight at 4 degrees

Blocking Agent

10% FBS

Secondary Incubation

Anti-rabbit Alexa-Fluor conjugated, 1:400 dilution, incubated for 1 hour at room temperature

Tertiary Incubation

None

Detection

Fluorescence microscopy

Results Summary

Used for immuno-fluorescent quantification of beta-cell area in embryonic pancreas. Signal is strong with little to no background, making downstream quantification via automated staining detection reliable and easy to optimize. Standard immunofluorescence staining protocol with heat-mediated antigen retrieval is sufficient to make antibody work well.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Strong staining at relatively high dilution

The Bad

No problems encountered after 1-2 years of use

The Bottom Line

Reliable antibody that requires little to no optimization. Works well on mouse pancreas paraffin sections with standard immuno-staining protocols.

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