Sep-Pak C18 to Purify Peptides

Havard Medical School
Dpt of Cell Bio
Postdoctoral Researcher

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Company:

Waters

Product Name:

Sep-Pak tC18 1 cc Vac Cartridge, 50 mg Sorbent per Cartridge

Catalog Number:

WAT054960

This product was used to buffer exchange peptides (digested proteins), prior to downstream mass spectrometry analysis.

Experimental Design and Results Summary

Application

Peptides subjected to C18 solid-phase extraction

Starting Material

Trypsin digested HeLa lysate

Tips

None

Results Summary

Peptides were repeatedly efficiently purified and buffer exchanged.

DOI or PMID #

10.1016/j.molcel.2018.03.012

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Summary

The Good

Ease of use

The Bad

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The Bottom Line

Bottom line is I use these C18 cartridge regularly and I'm very pleased with them for purifying peptide (unlabled or TMT labeled).

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