C18 Columns

C18 columns are liquid chromatography columns containing C18 hydrocarbon chains bound to a stationary matrix, generally silica. The longer chain length of C18 makes this media ideal for separating complex biomolecules such as peptides and small proteins. C18 columns are often used in reversed-phase HPLC to separate analytes in an aqueous, hydrophilic mobile phase from impurities like salts and buffers. When choosing a C18 column, consider the molecular weight of the target analytes and the intended chromatography conditions, such as flow rate, temperature, and pH range.