Cell Signaling Technology
LC3A
4599
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Browse Similar Products: LC3A Western Blot Antibodies
I work on selective forms of autophagy, especially focusing on ribophagy and ER-phagy. I have used large numbers of antibodies for proteins related to autophagy. This LC3A antibody is the second antibody i tried. This one works well.
Western Blot
HCT116 cells stably expressing GFP-LC3A
1:1000 dilution in 5% BSA, TBST
5% non-fat milk in TBST
1:20000 IRdye800 rabbit
Washed three times
Odyssey Clx
I tried an antibody from Abgent and it did not work in my hand. As a substitute, i ordered this antibody and it worked well. As indicated in the figure, it is quite selective for LC3A even though there's a 90% sequence homology with LC3B.
N/A
Selectivity
Nothing
Good for LC3A selectivity probing.