LC3A (D50G8) XP ® Rabbit mAb from Cell Signaling Technology

The LC3A (D50G8) XP® Rabbit mAb detects endogenous levels of total LC3A protein and may also react with LC3B. It has been shown to work in various applications, including Western blotting and immunohistochemistry, as evidenced by its use in studies such as those investigating autophagy mechanisms in different cell types and disease models, including cancer and respiratory conditions.

Yes, the LC3A antibody has been utilized in studies examining autophagy in various disease contexts. For instance, it was employed in research on T cell acute lymphoblastic leukemia to assess autophagy's role in metabolic reprogramming and therapeutic resistance (herranz2015). Additionally, it has been used in studies related to respiratory syncytial virus-induced asthma, highlighting its relevance in understanding autophagy in immune responses (chen2019).

The LC3A antibody is critical for detecting LC3 protein conversion, which is a hallmark of autophagy activation. Research has shown that the phosphorylation of LC3A by upstream signaling pathways, such as the Hippo pathway, is essential for autophagic flux and cell survival under stress conditions (seo2023). This highlights the antibody's role in elucidating the molecular mechanisms governing autophagy.

The LC3A antibody has been effectively used in Western blotting to analyze protein expression levels and in immunohistochemistry to visualize autophagy-related processes in tissue samples. For example, it has been cited in studies that assess the role of autophagy in various cellular contexts, including the examination of autophagic responses to therapeutic agents in cancer cells (ouyang2013).