Cell Signal Technology
4EBP1 total
9644
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I work on selective forms of autophagy, especially focusing on ribophagy and ER-phagy. I have used large numbers of antibodies for proteins related to autophagy. I use this 4E-BP1 antibody for mTOR activity measurement together with p-S6K. This antibody always works very well with high sensitivity
Western Blot
HCT116 and 293T cell lysate
1:3000 dilution in 5% BSA, TBST
5% non-fat milk in TBST
1:20000 IRdye800 rabbit
Washed three times before detection
Odyssey clx
This antibody is very strong. Sometimes each phosphorylated 4EBP1 cannot be resolved due to the too strong signal.
N/A
High sensitivity
Too high when lots of lysate is loaded
This is too good to work for large loading.