4E-BP1 (53H11) Rabbit mAb 9644S from Cell Signaling Technology

Product Specs
Item4E-BP1 (53H11) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number9644S
Quantity100 µl
ApplicationsW, W-S, IP, IHC-P, IF-IC, FC-FP
ReactivityH, M, R, Mk
Target4E-BP1
IsotypeIgG
HostRabbit
NCBI Gene Aliases4E-BP1, 4EBP1, BP-1, PHAS-I
NCBI Full Gene Nameeukaryotic translation initiation factor 4E binding protein 1
Clone53H11
Molecular Weight15-20
ClonalityMonoclonal
Research AreaTranslational Control

Questions and Answers

Can this antibody be used to study the effects of mTOR signaling in cancer cells?

Yes, the 4E-BP1 (53H11) Rabbit mAb has been utilized in studies investigating mTOR signaling pathways. For instance, it was shown that combining a PI3K inhibitor with mTORC1 inhibitors can enhance cell death in cancer cells, and the phosphorylation status of 4E-BP1 was a key factor in this response ().

Is this antibody effective for detecting 4E-BP1 in Western blotting experiments?

The 4E-BP1 (53H11) Rabbit mAb has been cited in multiple studies for use in Western blotting, demonstrating its effectiveness in detecting 4E-BP1 protein levels. For example, it was used at a dilution of 1:1000 in Western blot assays to assess protein signaling in various experimental contexts (, ).

What species can this antibody react with, and is it suitable for human studies?

The 4E-BP1 (53H11) Rabbit mAb is reactive with several species, including humans, mice, rats, and non-human primates. This broad reactivity makes it suitable for studies involving human samples as well as animal models (, ).

Has this antibody been used in any studies related to cancer progression?

Yes, the 4E-BP1 (53H11) Rabbit mAb has been referenced in studies examining cancer progression. For example, it was used to investigate the role of eEF2K in cancer cell viability, where its inhibition was linked to reduced tumor survival, highlighting the importance of protein synthesis regulation in cancer biology ().

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) Modulating starch digestion kinetics via feed processing: Implications for growth and metabolism in weaned pigsAnimal NutritionJune 1, 2026Nan Zhang, Shuang Dong, Shuyu Peng, Bing Dong, Crystal L. Levesque, Yongxi MaCell Signaling Technology, Danvers, MA, USA), eukaryotic initiation factor 4E-binding protein 1 (4E-BP1, catalog No. 9644, Cell Signaling Technology, Danvers, MA, USA), phosphorylated 4E-BP1 (p-4E-BP1, catalog No. 2855, Cell Signaling Technology

(2) Nebivolol suppresses glioblastoma progression via dual modulation of mitochondrial metabolism and AKT/mTOR/4EBP1 signaling axisPLoS GenetApril 24, 2026Lingni Zhou, Hongyu Che, Hongyan Jiang, Luxin Yin, Yongang Jiang, Yuhui Zhang, Haoran Liang, Rutong Yu, Xu Zhang, Xuejiao Liu(#12231), CDK6 (#3136), AKT (#9272), p-AKT (#4058), p-mTOR (#5536), mTOR (#2983), p-4EBP1 (#2855), 4EBP1 (#9644), GAPDH (#5174) and β-actin (#8457) primary antibodies were purchased from Cell Signaling Technology

(3) Cancer IDO1-Mediated Tryptophan-Kynurenine Metabolic Reprogramming to Drive Skeletal Muscle Atrophy and Cachexia AccelerationJ Cachexia Sarcopenia MuscleApril 24, 2026Leng Han, Lingjie Jing, Xinting Zhu, Ciqin Li, Lu Bai, Yang Fang, Yuxuan Zhou, Dingyuan Bai, Jin Lu, Yonglong Han, et al.Monoclonal Antibody (108D2, #9234), eIF4E‐binding protein 1 (4E‐BP1) Rabbit Monoclonal Antibody (53H11, #9644) and Phospho‐4E‐BP1 (Thr37/46) Rabbit Monoclonal Antibody (236B4, #2855) were purchased from Cell Signaling Technology

(4) Copper deficiency impairs oligodendrocyte maturation and social behavior via mitophagy and mTOR suppression in ASDSci AdvApril 3, 2026Noriyoshi Usui, Miyuki Doi, Stefano Berto, Kiwamu Matsuoka, Rio Ishida, Hana Miyauchi, Yuuki Fujiwara, Koichiro Irie, Michihiro Toritsuka, Takahira Yamauchi, et al.anti–phospho-S6 ribosomal protein (Ser240/244) (1:2000; #5364, Cell Signaling Technology), rabbit monoclonal anti–4EBP1 (1:1000; #9644, Cell Signaling Technology), rabbit monoclonal anti–phospho–4EBP1 (Thr37/46) (1:1000; #2855, Cell Signaling Technology

(5) GABARAPL1 is important for the activation of HRI during eIF2α phosphorylation-dependent stress response to sodium arseniteScientific ReportsMarch 28, 2026Sarah Campenet, Mathis Lamarre, Jules Durand, Valérie Perez, Paul Peixoto, Régis Delage-Mourroux, Michaël Guittaut, Aurélie Baguet, Gilles Despouy(Cell Signaling, #26632), polyclonal anti-HRI (Proteintech, 20499-1-AP), polyclonal anti-4EBP (Cell Signaling, #9644), monoclonal anti-HSP90 (Cell Signaling, #4877), polyclonal anti-ERK1/2 (Cell Signaling #9102), monoclonal

(6) Laptm4a mediates renal ischemia-reperfusion injury by regulating the UNC5B-AKT/mTOR signaling pathwayFrontiers in ImmunologyMarch 24, 2026Xiaoxiong Ma, Zhan Chen, Tianyu Wang, Zhongbao Chen, Tao Qiu, Jiangqiao Zhou,Merck Millipore,ABC89,1:1000 p-AKT,CST,4060,1:1000 AKT,Abclonal,A17909,1:1000 p-mTOR,CST,2971,1:1000 mTOR,Abclonal,A2445,1:1000 p-4E-BP-1,CST,2855,1:1000 4E-BP-1,CST,9644,1:1000 p-S6K,CST,9234,1:1000 S6K,GeneTex,GTX50304,1:1000 β-actin,Abclonal,AC026,1:10000 Information

(7) Legionella Lem26 functions as an ATG8-activated effector that inhibits host autophagymBioMarch 11, 2026Kevin R. Parducho, Zi Yang, Emily Guinn, Daniel Choi, Shanta Nag, Thomas J. Melia, Craig R. Royanti-phospho-S6 Ribosomal Protein (Ser 235/236) (Cell Signaling #4858, 1:1,000), anti-4E-BP1 (Cell Signaling #9644, 1:1,000), and anti-pan-ADP-ribose binding reagent (Millipore Sigma MABE1016, 1:1000) for 1 h at RT

(8) Neurons with granulovacuolar degeneration bodies are resilient to tau-induced protein synthesis impairmentSci AdvMarch 6, 2026Jasper F. M. Smits, Thijmen W. Ligthart, Marta Jorge-Oliva, Skip Middelhoff, Fleur Schipper, Débora Pita-Illobre, Ka Wan Li, Wiep Scheperpolyclonal,1:1000,,Synaptic Systems,101004 4E-BP1,,Rabbit monoclonal,,1:1000,Cell Signaling Technology,9644 p-4E-BP1,Thr37/Thr46,Rabbit monoclonal,,1:1000,Cell Signaling Technology,2855 Antibodies used in

(9) Eight-week dietary methionine restriction does not impair resistance exercise-induced mTORC1 signalling activation in ratsBiochemistry and Biophysics ReportsMarch 1, 2026Naoki Fukao, Mito Watanabe, Ryo Takagi, Koki Okumura, Naomi Yoshii, Koma Kawabata, Satoshi FujitaPhospho-p70S6K Thr389 (#9205, CST), rpS6 (#2217, CST), Phospho-rpS6 Ser240/244 (#2215, CST), 4E-BP1 (#9644, CST), Phospho-4E-BP1 Thr37/46 (#2885, CST), Puromycin (#MABE343, Merck Millipore, Burlington, MA, USA),

(10) A cellular basis for the mammalian nocturnal-diurnal switchScienceFebruary 26, 2026Andrew D. Beale, Matthew J. Christmas, Nina M. Rzechorzek, Andrei Mihut, Aiwei Zeng, Christopher Ellis, Nathan R. James, Nicola J. Smyllie, Violetta Pilorz, Rose Richardson, et al.rabbit anti-S6K pT389 (Cell Signaling, #9234S, RRID:AB_777802; 1:1000), anti-4EBP1 (Cell Signaling, #9644S, RRID:AB_2097841 ; 1:1000), rabbit anti-4EBP1 pT37/T46 (Cell Signaling, #2855S, RRID:AB_560835; 1:1000),

(11) Eukaryote initiation factor 6 modulates small-cell lung carcinoma plasticity via the integrin-FAK signaling axisNature CommunicationsFebruary 24, 2026Haoning Peng, Zhile Wang, Mengyao Wang, Zheyu Ding, Kaixiu Li, Yuqing Wang, Xuejiao Yu, Siyang Song, Yulan Deng, Yi Liu, et al.anti-phospho-p70 S6 Kinase (Thr389) (108D2) (CST #9234, USA, 1:1000, RRID: AB_2269803), rabbit anti-4E-BP1 (53H11) (CST #9644, USA, 1:1000, RRID: AB_2097841), rabbit anti-phospho-4E-BP1 (Ser65) (CST #9451, USA, 1:1000, RRID: AB_330947),

(12) Phosphoproteomics identification of ERK-dependent activation of Rps6kb1 in cardiac hypertrophyJCI InsightFebruary 23, 2026Chao Li, Pengfei Zhang, Kai Zhang, Jane A. Cook, Weidan Song, Megan Virostek, Lily A. Slotabec, Nadiyeh Rouhi, Mohammed Hazari, Michael I. Adenawoola, et al.(Cell Signaling Technology, 5364), p-S6 S235/S236 (Cell Signaling Technology, 4858), 4EBP1 (Cell Signaling Technology, 9644), p-4EBP1 T37/T46 (Cell Signaling Technology, 2855), Myc (Santa Cruz Biotechnology, sc-40), HA (Cell Signaling Technology

(13) Non-enzymatic ABHD6 interacts with Akt-FoxO1 axis to regulate selective hepatic insulin resistancebioRxivFebruary 17, 2026Guannan Li, Laurence T. Maeyens, Jiyuan Yin, Jan-Bernd Funcke, Chanmin Joung, Ruizhen Li, Ziying Xu, Ting Wu, Xin Li, Nisi Jiang, et al.#5536, 1:1000), mTOR (CST, #2972, 1:1000), phospho-4E-BP1 (Thr37/46) (CST, #2855, 1:1000), 4E-BP1 (CST, #9644, 1:1000), LSD1 (CST, #2184, 1:1000), β-Actin (Abclonal, AC004, 1:10,000), and GAPDH (Abclonal, AC002,

(14) Hyperactivation of mTORC1 signaling mediates folliculin deficiency-induced pulmonary cyst formation in Birt-Hogg-Dubé syndromeJ Clin InvestFebruary 16, 2026Ke Cao, Hui Chen, Ling Chu, Hong-Jun Wang, Jianhua Zhang, Yongfeng Luo, Joanne Chiu, Damir Khabibullin, Nicola Alesi, Matthew E. Thornton, et al.anti-GAPDH, pS6, S6, phospho-4E-BP1, 4E-BP1, RAPTOR, and mouse anti–β-actin (2118, 2211, 2217, 2855, 9644, 48648, 3700 from Cell Signaling Technology) and rabbit anti-FLCN antibody (a gift of Arnim Pause at

(15) A CDK-4EBP1 signaling axis drives HSV-1 replication and underscores a druggable pathway for potent antiviral interventionmBioFebruary 11, 2026Krishnaraju Madavaraju, Tejabhiram Yadavalli, Sudhanshu Kumar Singh, Chandrashekhar D. Patil, Hemant Borase, Deepak ShuklaUSA",26 Phospho-4E-BP1 (Thr37/46),236B4,"Cell Signaling Technologies, USA",15 to 20 4EBP1 (53H11),#9644,"Cell Signaling Technologies, USA",15 to 20 HDAC1,A19571,"ABclonal, USA",~70 Cell cycle phase determination

(16) Identification of Raptor and GLI1 as USP37 substrates highlight its context-specific function in medulloblastoma cellsOncogeneFebruary 10, 2026Ashutosh Singh, Donghang Cheng, Amanda R. Haltom, Yanwen Yang, Tara Dobson, Rashieda Hatcher, Veena Rajaram, Vidya Gopalakrishnanincubated with control mouse IgG, anti-USP37 (Cat# A300-927A, Thermo Fisher Scientific), anti-4EBP1 (Cat# 9644, Cell Signaling Technology, MA, USA), anti-eIF4E (Cat# 2067, Cell Signaling Technology) primary antibodies

(17) p53 Drives Lung Cancer Regression through a TSC2/TFEB-Dependent Senescence ProgramCancer DiscoveryFebruary 6, 2026Mengxiong Wang, Kathryn T. Bieging-Rolett, Alyssa M. Kaiser, Colleen A. Brady, John H. Lockhart, Sofia Ferreira, Kha T. Nguyen, Arati Rajeevan, Simone A. Evans, Tianyu Zhao, et al.cat. #BML-PW9860, RRID: AB_2196009; 1:1,000), rabbit anti–4E-BP1 (Cell Signaling Technology, cat. #9644, RRID: AB_2097841; 1:1,000), rabbit anti–phospho-4E-BP1 (Thr70; Cell Signaling Technology, cat. #9455,

(18) Disruption of FGL2 induces TFEB-dependent lysosomal degradation of PD-L1 and enhances the efficacy of anti-PD1 therapy in hepatocellular carcinomaCell Communication and SignalingFebruary 2, 2026Meiwen Han, Qianting Guan, Fangfei Yan, Ming Wang, Dong Xi, Qin Ning(17168-1-AP, Proteintech), p70S6K (1:1000) (2708, CST), phospho-p70S6K (1:1000) (9234, CST), 4EBP1 (1:1000) (9644, CST), phospho-4EBP1 (1:1000) (2855, CST), AKT (1:1000) (9272, CST), phospho-AKT (1:1000) (4060, CST),

(19) A dose-dependent switch in translation capacity controls the transcription factor response to H 2 O 2 stressbioRxivJanuary 31, 2026Chance Parkinson, Woody March-Steinman, Elizabeth Jose, Lisa Shanks, Andrew L. Paek-Tuberin/TSC2 (D93F12) XP® rabbit mAb from Cell Signaling Cat# 4308S (1:1000). Anti-4E-BP1 (53H11) rabbit mAb from Cell Signaling Cat# 9644S (1:1000). Secondary antibodies used: IRDye® 800CW Goat anti-Mouse IgG from LICORbio Cat# 926-32210 (1:20,000).

(20) Ad-VT Oncolytic Adenovirus Suppresses Bladder Cancer via cAMP-Dependent AMPK-Raptor Activation and G2/M ArrestTumour Virus ResearchJanuary 29, 2026Dapeng Li, Jing Lu, Ran Zhu, Xianyan Sun, Cuiling Zhang, Mingzhe Sun, Chengyuan Ma, Chao Shang, Xiao LiRaptor (#2280), p-Raptor (#2083), mTOR (#2983), p-mTOR (#5536), S6 (#2317), p-S6 (#4858), 4E-BP1 (#9644), p-4E-BP-1 (#2855), and GAPDH (#5175) were purchased from Cell Signaling Technology. CAR(Cat No. 86186-1-RR)

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9/24/2019Super Strong 4E-BP1 Antibody from CSTDr. H. AnI use this for mTOR activity measurement together with p-S6K. This antibody always works very well with high sensitivity.

4E-BP1 (53H11) Rabbit mAb

4E-BP1 (53H11) Rabbit mAb from Cell Signaling Technology

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4E-BP1 (53H11) Rabbit mAb from Cell Signaling Technology

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