Cell Signaling Technology
ATG7
8558
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I work on selective forms of autophagy, especially focusing on ribophagy and ER-phagy. I have used large numbers of antibodies for proteins related to autophagy. This Atg7 antibody is very specific and works well
Western Blot
293T, 293, HCT116 cells
1:1000 overnight incubation
5% non-fat milk
1:20000 odyssey IRDye800, 1h
TBST wash three times, 5 min
Odyssey Clx
The antibody works very well, and it is validated by KO cells.
10.1016/j.molcel.2019.03.034
N/A
Specificity
Nothing
I recommend this antibody to others working in the autophagy field