A Good Smo Antibody from Proteintech

Sir William Dunn School of Pathology
Oxford University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Proteintech

Product Name:

Smo rabbit antibody

Catalog Number:

20787-1-ap

Clone Number:

Polyclonal

This antibody can be used for the cilia localisation staining of Smo (IF), and also works for detecting Smo at the protein level (WB).

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human RPE1

Primary Incubation

1:1000 in 1% milk in PBST at 4 degree overnight

Blocking Agent

1% milk in PBST

Secondary Incubation

1:5000 in 1% milk in PBST at room temperature for 1 hour

Tertiary Incubation

N/A

Detection

ECL

Results Summary

It recognised endogenous Smo at ~ 110 kD, and that band was sensitive to RNA knockdown of Smo.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Specific

The Bad

Some nonspecific bands

The Bottom Line

A good antibody for studying Smo.

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