Novus TRF-2 Antibody Works well!

Internal Medicine
East Tennessee State University
Lab Technician

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Company:

Novus Biologicals

Product Name:

TRF-2 Antibody (4A794.15) [FITC]

Catalog Number:

NB100-56506F

Clone Number:

4A794.15

To see how KML001 affect TRF2 expression in CD4 T cells. CD4 T cells were cultured with KML001 for 2 days, then harvested, permeabilized with Fixation/Permeabilization Concentrate and Diluent, then stained TRF2-FITC (1:50) in 1x Permeabilization buffer for 40 min, washed, applied on Flow Cytometry.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human CD4 T cells

Primary Incubation

1:50, 40mon

Blocking Agent

Isolation buffer

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD C6 Plus

Results Summary

TRF2 still expression after treated with KML001, not completely inhibited.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Separate well!

The Bad

None found.

The Bottom Line

Works well for CD4 T cells.

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