Sigma THSD7A Antibody Works on Kidney

August 05, 2019

BlueskyReddit
Pathology
Johns Hopkins University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Sigma Aldrich

Product Name:

Anti-THSD7A antibody produced in rabbit

Catalog Number:

HPA000923

Clone Number:

Polyclonal

We studied PLA2R-negative membranous nephritis kidney biopsies, and it turned positive in some of them.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Human Kidney Biopsy

Primary Incubation

1:800 for 45 minutes

Blocking Agent

Peroxidase blocker

Secondary Incubation

Poly Link- Poly HRP

Tertiary Incubation

None

Detection

DAB chromogen

Results Summary

It turned positive in some of the PLa2R-negative membranous nephritis cases, and can be used as a marker to identify primary membranous nephritis cases.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Good discrimination of the positive and negative cases

The Bad

Nothing

The Bottom Line

Note: We used EDTA for Ag retrieval.

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